Gintama anime always have different story in each chapter. It finds that the "Yorozuya" themes can make all things happen in this anime. In the chapter 139 - 146, Sakata Gintoki and his Yorozuya team make their move on the underground city called Yoshiwara. In these chapters, there are some new appearance of new character. We can say the Yoshiwara protector, Tsukuyo, and then Hinowa, Yoshiwara 'sun' and his son, Seita. Also performing some action about Yato clans, the Yoshiwara leader, Hosen, and Kagura's sister Kamui.
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